T84 human intestinal epithelial cells primarily grow as a monolayer with intact tight junctions (a & b) which are
preserved during cell division (c). T84 cells stably expressing an occludin anti-sense construct under the constitutively
active CMV promoter show disrupted junctions and epithelial morphology (d-f). Note that anti-sense cells lacks contact-inhibition
of growth and continues to divide at all layers of the multilayer dysplasic epithelium. Arrows point to cells in mitosis.
Arrowheads point to lack of tight junction in anti-sense cells (g & h).
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