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EC1 peptide has non-competible covaspheres binding activity suggesting multiple binding sites or polymerization. Negative staining of occludin extracellular domain 1 peptide confirms that it polymerizes to form fibrils in solution.
vw1fresh.jpg
Extracellular domain 1 peptide freshly prepared as 10 mM solution (click picture to enlarge).
vw12weeks.jpg
Extracellular domain 1 peptide as 10 mM solution allowed to incubate at room temperature for 2 weeks (click picture to enlarge).
vw1months.jpg
Extracellular domain 1 peptide as 10 mM solution allowed to incubate at room temperature for months (click picture to enlarge).

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